Selective Detection by Dpv of Benserazide and Levodopa in Pharmaceutical Preparates

For the detection of some important biological substances by electrochemical methods the carbon modified electrodes have been widely used [1-3]. The aim of this work was to study the simultaneous detection of benserazide and levodopa in pharmaceutical tablets of MADOPAR^® by the voltammetric method using a modified C-PVC electrode. The preparation and modification of the C-PVC electrode was performed according to the previously reported method [4]. The tablet extract in presence of excipients of MADOPAR^® in phosphates buffer pH 7.4 was used for the measurements without pretreatment. The electrochemical experiment by VDP of the MADOPAR^®extract using the C-PVC modified electrode showed good defined oxidation peaks for benserazide at 24 mV and levodopa at 141 mV. The oxidation current was proportional to the volume of the extract in the buffer solution (Fig.1). The presence of an excess of ascorbic acid (140 µM) did not affect the detection of benserazide and levodopa. The modified C-PVC electrode can be used for the simultaneous determination of benserazide and levodopa in some real samples at different pH values of buffer solutions.

References

[1] J.B. Raoof, R. Ojani, M. Amiri-Aref, M. Baghayeri; Sens. Actuators, B. 166–167 (2012) 508–518.

[2] EB Sakineh, B Hadi, KM Hassan, SN Somayeh, SN Vahhab, R Sara; J Anal Meth Chem 2012 (2012) 1-8.

[3] Mu Naushad, VK Gupta, SM Wabaidur, ZA Alothman; Int J Electrochem Sci. 8 (2013) 297-311.

[4] P.Ruiz-G, M.P. Elizalde-G, M.M Davila, Esp. Pat., Appl. P201430231 (2014).

Acknowledgments

We acknowledge the financial support of CUVyTT-BUAP under project No.014.