Determination of the Antioxidant Capacity of "Pistache Amargo" Using a Biosensor Based on Laccase Trametes Versicolor

In the Isthmus of Tehuantepec region of Oaxaca, Mexico, it grows a variety of Pistache of the Anacardiaceae family; the fruit comprises about 68 % weight oil and is rich in polyphenolic compounds, which are antioxidant molecules of great interest today for their beneficial implications to human health, as presently there is a strong interest in finding natural sources of antioxidants. Therefore, this work presents the spectrophotometric studies using the DPPH radical method for the determination of the antioxidant capacity equivalent to caffeic acid in different extracts of the residual pulp "Pistache Amargo". The spectrophotometric method is contrasted with an electrochemical method using a biosensor based on the enzyme Laccase Trametes Versicolor immobilized on a polymeric membrane of polyvinyl alcohol.