Fabrication of Electrochemical Biosensor for Cholesterol Using CNT-Gold Nanohybrid Buckypaper
For this cholesterol biosensor, Cholesterol oxidase has been immobized on AuNP modified buckypaper. In addition to that buckypaper with carboxyl functionality was also modified by 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide, (EDC) and N-hydroxy succinimide(NHS) and after this activation cholesterol oxidase was immobilized. These two series of Buckypaers were used as electrodes. biosensor.
Cholesterol was dissolved in 10mM tritobn X and different solution of cholesterol were made using 0.1 M phosphate buffer solution. Cyclic voltametry, amperometry and differential pulse voltammetry techniques wesr used to study the electrochemical behavior of this biosensor and to determine the sensitivity. Above figures show DPV results and the calibration plot obtained for cholesterol solution concentration ranging from from 0.2-1mM/L using AuNP –MWCNT hybrid buckypaper/. A detection limit obtained was 3 mmole/L which is higher than many reported biosensors based on other CNT dispersion based biosensors.
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