In a previous work, the ability of 2-hydroxyethylammonium formate ionic liquid to act as an “all-in-one” reaction medium was tested for Pd and Pd-NiO nanoparticles. In this work, the proof of its power to synthesize metallic nanoparticles was extended to the following materials: Au, AuPd, AuPt and AuCuO nanoparticles as well as Pd and CuO as controls. These materials were used as electrocatalysts for the glucose electro-oxidation reaction. According with XRD, crystallite sizes of 16.9, 11.8, 12.0, 12.6, 10.1 and 11 nm were obtained for Au/C, AuPd/C, AuPt/C, AuCuO/C, Pd/C and CuO/C, respectively. TEM micrographs showed nanoparticle sizes closed to crystallite sizes. For metallic mixtures, X-ray fluorescence revealed metallic compositions of Au₆₀Pd₄₀, Au₉₀Pt₁₀ and Au₈₅CuO₁₅. Moreover, thermogravimetric analyses showed metal mass contents between 24 to 32 wt. % (Vulcan carbon was used as support) for all materials except AuCuO which showed 4 wt. %. The electrocatalytic activity toward glucose electro-oxidation was tested as function of its concentration and temperature. All materials showed electrochemical response to the presence of glucose. For fuel cell applications, AuPt showed the most negative oxidation potential (-0.57 V vs. NHE at 40°C) and the highest peak current density (58.5 mA mg^-1). For electrochemical non-enzymatic glucose detection, all materials showed good response to the successive addition of 1 mM glucose at short times (less than 10 seconds). However, due to its easiness to be synthesized, and its relative low cost, CuO/C can be an attractive option for non-enzymatic glucose sensors.