Platelet-derived growth factor (PDGF) are proteins that play an important role in the regulation of tumour growth and division. PDGF has been associated with cancer and other proliferative disease states, such as atherosclerosis and glomerulonephritis. However, as a potential cancer marker, PDGF is difficult to detect due to its trace amount in real blood sample. Recently developed Electrochemical Proximity Assay (ECPA) is an extremely flexible and sensitive technique with high potential for quantitation of a variety of proteins in clinical laboratory settings or at the point-of-care systems. Here, a newly designed Multi-Parametric/Multimodal Spectroscopy Apparatus techniques was used for PDGF sensitive detection. With the combined advantages of three surface characterization techniques such as ECPA, surface enhanced fluorescence and surface plasmon resonance spectroscopy, the sensitivity of PDGF quantification was significantly improved with potential to reach as low as fmol level. Additionally, the mechanisms of action and dynamics of sensing and/or protein recognition in the process of ECPA was studied.