Cholesterol efflux from the cell plasma membrane is known to be increased in cell-line and mouse models of cystic fibrosis.  A noninvasive means of characterizing plasma membrane cholesterol efflux at affected tissues is needed to extend the trends found models of cystic fibrosis to humans suffering from the disease.  Microelectrode induced cholesterol efflux from the plasma membrane surface of tissue is proposed as a strategy to demonstrate increased cholesterol efflux for cystic fibrosis in human subjects.  Demonstration for electrochemical sensor detection of cholesterol efflux from the human buccel mucosa has been accomplished by this research group.  Using a pulsed potential scheme for detection of hydrogen peroxide, cholesterol oxidase modified platinum microelectrodes show proof-of-concept for the in vivo diagnostic assay.  Increased signal has been achieved using carbon microelectrodes containing electrodeposited platinum crystallites.  Additional optimization of the sensor is being explored by synthesizing platinum crystallites in solution for subsequent deposition on the carbon microelectrode.  The aim is to improve repeatability of sensor fabrication by better controlling the size, shape, and distribution of platinum crystallites deposited on the carbon microelectrode surface.