Since Clark and Lyons explained the basic concepts of glucose enzymatic electrodes, there have been significant efforts on development of reliable devices for diabetes control. This metabolic disorder rooted in insulin deficiency which results in blood glucose concentrations lower or higher than normal range of 4.4 to 6.6 mM. The primary approach to develop electrochemical glucose sensors is to exploit enzymes to oxidize glucose and monitor hydrogen peroxide production. The complications associated with enzyme immobilization and the necessity for maintaining certain operating and storage condition (controlling temperature, pH and humidity) along with the enzymes high cost lead to the development of non-enzymatic glucose sensors (NEG). In NEG sensors, glucose is oxidized directly on electrode surface. In this study, a simple design disposable NEG sensor with high sensitivity and acceptable interference rejection was developed based on NiP/PET electrode. An activation-free Ni electroless metallization was performed using (3-Aminopropyl)trimethoxysilane (APTMS) and (3-Mercaptopropyl)trimethoxysilane (MPTMS) self-assembled monolayers (SAM). The affinity of –HS functional groups on MPTMS-SAM to Ni resulted in a thin film with excellent adhesion. A PMMA mask was used during Ni electroless deposition to obtain a 3-electrode configuration pattern on plastic substrate. Pt electrodeposition was carried out on two of the three Ni electrodes to serve as reference and counter electrodes. Nafion solution was drop-casted on Ni working electrode to reject interference. Electrochemical measurements were carried out in 0.1M KOH solution. 0.5 V was selected as the sensor working potential base on cyclic voltammetry characterizations in presence of glucose. Chronoamperometric measurements presented a wide linear range with high sensitivity of 230 µA.mM^-1.cm^-2. Results of interference studies proved the accuracy of sensor response in presence of ascorbic acid, uric acid and lactate (less than 15% error which is considered as clinically accurate). The study suggests a low-cost and high performance nonenzymatic glucose sensor compatible with roll to roll manufacturing and mass production.