Increasing energy demands, depletion of fossil fuel resource and environmental pollution require the researches of alternative fuel as well as energy conversion devices. Proton Exchange Membrane Fuel Cells (PEMFCs), available applied for emergency and/or transport power supply, are the systems in which hydrogen is used as the fuel for anode. In this study, we design a combined system of PEMFC stack operating with biohydrogen as fuel and pure oxygen or air as oxidant. The biohydrogen is produced from a raw bamboo biomass treatment process. This process conclude two steps (i) advanced oxidation and (ii) fermentation.