Nanomaterials are emerging as attractive platforms for enzyme immobilization for various electrocatalytic applications^1-3. The superior electronic properties and large surface area of such nanomaterials gained significant attention in developing enzyme electrocatalytic systems^4-5. In this study, we have prepared and characterized multiwalled carbon nanotube based “flexible” bucky papers for enzyme electrocatalysis. We have prepared bucky papers with different thicknesses ranging from 80 to 400 μM using the vacuum filtration method⁶. They are characterized by microscopy and electrochemistry for surface morphology and for electrochemical properties including conductivity and capacitance. These bucky papers are further immobilized with bilirubin oxidase enzyme to perform direct oxygen reduction without any redox mediators. Additionally, applicability of these bucky papers for membrane bound heme-enzyme catalysis is under examination. Our findings on this project will be presented.

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