Hydrogels have emerged in the biomedical field as alternatives to traditional wound dressings due to their well-defined structure and intrinsic properties such as swellability and biocompatibility. These supramolecular nanocomposites can be modified with various additives including metal ions, organic crosslinkers, antimicrobial agents, and conductive polymers to enhance these properties as well as promote responsive characteristics. This research focuses on the development of a nanocomposite to monitor the properties (oxygen diffusion and antimicrobial effectiveness) and lifetime (swellability) of a hydrogel in real-time. More specifically, this nanocomposite is a multi-layered system in which the first layer consists of a biomimetic, alginate hydrogel containing functionalized metal nanoparticles (MNPs). The MNPs role is two-fold: to provide structural rigidity with enhanced electron transport and serve as a solid support for the functionalization of antimicrobial agents at the wound surface. The top scaffold of the composite is the bulk conductive polymer matrix that serves as the signal-response layer (i.e. receives charge from the biomimetic layer). Initial results show, as a simulated wound secretes, ions diffuse through the biomimetic hydrogel into the bulk conductive layer increasing the electron density to enhance the conductivity via p-doping. The changes in voltage and current can be used to track the integrity of the matrix as well as properties such as oxygen diffusion and antimicrobial effectiveness. Furthermore, results indicate a strong correlation between hydrogel matrix porosity and electrical responsiveness of the resultant nanocomposite material.