To explore the efficiency of pure propilase (PP) and the nano-emulsion formulation of propilase (PNE) as natural decontaminants against the fungal growth and the released mycotoxins, samples of infant baby food and their sputum in cases suffered from chronic respiratory diseases were examined. The incidence and frequency of A. flavus isolates growth showed moderate percentages of contamination in investigated samples. TLC/UV screen analysis confirmed aflatoxins production (61.8 %) of A.flavus strains isolated from sputum and (63.2%) of infant food strains. HPLC detection for control samples revealed positive contamination of AFB1, AFB2 and AFG2 levels at 0.2541± 0.26, 0.0048 ± 0.430 and 0.0007± 0.07 mg/kg, respectively. Synthesized propolis nano-emulsion (PNE) have been prepared by our team with some novel effective modifications, and newly compound with specific physical and chemical properties was obtained. The areas of inhibition were recorded as antifungal activity with a significant difference between PP and PNE. PP and PNE revealed a significant decrease in AFB1, AFB2 and AFG2 concentrations than the control. This study promotes to use the natural antioxidant and their nano- formulation to increase the level of food safety.