We describe the electrochemical detection of single nanoparticle (NP) collision at an ultramicroelectrode (UME) with various NPs such as Pt, Ag, Cu, and Ni NP. We could observe the single NP collision through the enhanced current by electrocatalytic redox recycling. The overall current transient caused by the NP collision was differed depending on the NP collision system. The peak height and frequency of the current response are proportional to the size and concentration of the NP. The current response is very sensitive to the material and surface state of the measuring electrode. It also changed depending on the applied potential and the reaction mechanism of collided NP. Therefore, the current response was investigated at the various electrode material and surface state of the measuring electrode for better understanding of single NP collision mechanism and NP’s electrocatalytic reaction. For Pt and Ag NPs, the change of current response was mainly investigated with various experimental conditions. For Cu and Ni NPs, the new electrocatalytic reaction such as glucose oxidation and urea oxidation were applied. By observing and analyzing the various current response of the Pt, Ag, Cu, and Ni NP’s collision, the electrocatalytic activity of NP at various circumstance at a single NP level was studied.