Nitric Oxide (NO) is an essential component in cell signaling, but its concentration and mechanisms of action during healthy and diseased states is largely unknown. Researchers have tried to quantify NO for years, but the lack of a sensor that can detect NO, not an upstream or downstream indicator of NO’s presence, in a spatial format has hindered their ability. Through the use of single walled carbon nanotube sensors that are adhered to a sterile platform, the extracellular concentration of NO can be quantified in a spatial-temporal manner. Mapping the concentration of NO for different cell types as they perform different functions, specifically proliferation and migration, will allow for a greater understanding of cell function and the development of more effective treatments of diseased cells in the future.