Wednesday, 1 June 2022: 08:20
West Meeting Room 209 (Vancouver Convention Center)
Microelectrochemistry has a long history in the life sciences for in vivo and in vitro measurements of
e.g., pH, oxygen, and signaling molecules such as nitric oxide (NO), adenosine-5`-triphosphate (ATP),
hydrogen peroxide (H2O2) and neurotransmitters. In particular, gaining information on signaling
molecules at live cells, e.g., during and after stimulation is of significance for understanding cell
signaling. Within recent years, our research team has focused on the development of micro-sized
sensing approaches for the detection of ATP [1,2], which is considered among the most important
autocrine and paracrine signaling molecules [3]. H2O2 belongs to the group of reactive oxygen species
(ROS), which is in contrast to radical ROS rather stable. H2O2 is involved in many physiological
processes such as hypoxic signal transduction, cell differentiation and proliferation but is also involved
in mediating immune responses [4]. The effect is, among other parameters, dependent on its local
concentration and its exposure time.
Platinum black is a highly suitable electrocatalytic nanomaterial for the electrochemical detection of
H2O2 [5]. We recently demonstrated that Pt black modified microelectrodes can be used for H2O2 at
granulocytes and peripheral blood mononuclear cells from pigs [6]. Within this presentation, we will
introduce dual functional miniaturized probes that allow further miniaturization of electrochemical
sensors for biomedical applications. We will demonstrate that these probes have great potential for cell
measurements, i.e., at macrophages that produce and release ROS in response to phagocytosis or
stimulation.
e.g., pH, oxygen, and signaling molecules such as nitric oxide (NO), adenosine-5`-triphosphate (ATP),
hydrogen peroxide (H2O2) and neurotransmitters. In particular, gaining information on signaling
molecules at live cells, e.g., during and after stimulation is of significance for understanding cell
signaling. Within recent years, our research team has focused on the development of micro-sized
sensing approaches for the detection of ATP [1,2], which is considered among the most important
autocrine and paracrine signaling molecules [3]. H2O2 belongs to the group of reactive oxygen species
(ROS), which is in contrast to radical ROS rather stable. H2O2 is involved in many physiological
processes such as hypoxic signal transduction, cell differentiation and proliferation but is also involved
in mediating immune responses [4]. The effect is, among other parameters, dependent on its local
concentration and its exposure time.
Platinum black is a highly suitable electrocatalytic nanomaterial for the electrochemical detection of
H2O2 [5]. We recently demonstrated that Pt black modified microelectrodes can be used for H2O2 at
granulocytes and peripheral blood mononuclear cells from pigs [6]. Within this presentation, we will
introduce dual functional miniaturized probes that allow further miniaturization of electrochemical
sensors for biomedical applications. We will demonstrate that these probes have great potential for cell
measurements, i.e., at macrophages that produce and release ROS in response to phagocytosis or
stimulation.
References
[1] E. Hecht, A. Liedert, A. Ignatius, B. Mizaikoff, C. Kranz, Biosens. Bioelectron. 2013, 44, 27.
[2] C. Ziller, et al., ChemElectroChem 2017, 4, 864.
[3] R. Corriden, P. A. Insel, Sci. Signal. 2010, 3, 104.
[4] C. Lennicke et al., Cell Commun. Signal. 2015, 13, 39.
[5] Y. Li, C. Sella, F. Lemaitre, M.G. Collignon, L. Thouin, C. Amatore, Electroanalysis 2013, 25, 895.
[6] A. Hellmann, S. Daboss, F. Zink, C. Hartmann, P. Radermacher, C. Kranz, Electrochim. Acta 2020, 353, 30851