An electrochemical sensor for nitrite detection has been prepared using novel positively charged nanocomposite consist of gold nanoparticles (AuNPs), poly [2-(methacryloyloxy) ethyl]trimethylammonium chloride (PMTC) and nano size ball milled jute carbon (JC). This report demonstrates the electrocatalytic property of the nanocomposite-modified glassy carbon electrode (AuNPs-PMTC-JC/GCE) to oxidized nitrite at lower potential. Characterization of surface morphology and elemental composition of the novel composite material was evaluated by performing ultraviolet-visible spectroscopy (UV), field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM), selected area energy diffraction analysis (SADP), X-ray diffraction analysis (XRD) and energy dispersive spectroscopy (EDS). Zeta potential analysis also carried out to find the charge property of the composite surface. Scan rate and pH analysis helped to bring out the kinetic involved in oxidation of nitrite at AuNPs-PMTC-JC/GCE. Calculated kinetic parameters were further used for computational CV simulation. Quantitative analysis was carried out by using CV and amperometric I-t analysis. In I-t analysis, the LOD (S/N = 3) value was calculated 0.22 µM within a linear range 0.94 -663.69 µM and in case of CV 1.28 µM LOD value was obtained within a wide range 10µM-1mM. The modified electrode showed impressive storage stability, data reproducibility and inti-interference effect against common interferents. CV response was observed for real sample analysis performed in tap water containing fixed amount of nitrite and compared the result with the standard solution.