Tuesday, 11 October 2022: 10:00
Room 308 (The Hilton Atlanta)
Although quantitative and qualitative electrochemical measurements of Reactive Oxygen Species (ROS) have been reported in the literature [1,2], real-time measurements of reactive oxygen species released from living organisms remains difficult, because they are unstable and released in trace amounts making their quantification challenging. Herein, we present a reliable, user-friendly, cost-effective and time-efficient method to fabricate micro-optical-ring electrodes (MOREs) [3], which enables the detection of ROS species close to the surface of biological samples. Electrochemical characterization, numerical simulations and Scanning Electron Microscopy (SEM) of MOREs reveal electrodes with a well-defined geometry and accurate experimental current signals. As a proof of concept, we demonstrate the use of MOREs as Scanning Photoelectrochemical Microscopy (SPECM) probes to study oxygen production in algae. This presentation highlights the capability of MOREs to detect species flux from single living cells, both spectroscopically and electrochemically, to potentially monitor the release of ROS from biological entities and providing information about disease initiation and progression.
[1] Y. Li, K. Hu, Y. Yu, S.A. Rotenberg, C. Amatore, M. V. Mirkin, Direct Electrochemical Measurements of Reactive Oxygen and Nitrogen Species in Nontransformed and Metastatic Human Breast Cells, J. Am. Chem. Soc. 139 (2017) 13055–13062.
[2] K. Hu, Y. Li, S.A. Rotenberg, C. Amatore, M. V. Mirkin, Electrochemical Measurements of Reactive Oxygen and Nitrogen Species inside Single Phagolysosomes of Living Macrophages, J. Am. Chem. Soc. 141 (2019) 4564–4568.
[3] N. Thomas, N. Ahmed , V. Singh, D. Trinh, S. Kuss, Micro-Optical-Ring Electrodes and their Application to Scanning Photoelectrochemical Microscopy for Single Live Cell Imaging. 2022 (submitted).