Nonaqueous Lithium-air (Li-O₂) battery has attracted significant research interest in the past decade, because of its high theoretical specific energy far beyond conventional Li-ion battery. However, there are many challenges such as severe degradation during cycling that limits the practical utilization of Li-O₂ battery. Hence, a powerful tool is necessary to be found to detect the intermediates and study side reactions in Li-O₂ battery. In this work, we explored a method to fabricate larger-area regularly patterned gold nano-dots by using the mask of anodic aluminum oxide (AAO) membrane for surface enhanced Raman spectroscopy (SERS).  The size effect of the small and large gold nano-dots on the enhancement of Raman signal was studied by Rhodamine 6G on Si. Small gold nano-dots on Au film and graphene were used as SERS-active cathodes in Li-O₂ battery and showed great ability for detecting the component on planar electrode. After discharge, many peaks have been observed for the SERS-active gold electrode, which demonstrate the existence of Li₂O_2, LiOH and LiO₂. On the other hand, when SERS-active graphene electrode was used as lithium oxygen battery cathode, Li₂O₂ and Li₂CO₃ were detected.