The Selective Coupling and Quantification of Peptides on a Polymer-Coated Microelectrode Array

Microelectrode arrays have been utilized by our group for several years to monitor the binding event of a biological system in “real-time”.¹ The use of a diblock co-polymer coating over the electrode surface as a support structure for the molecules of interest has been crucial to the success of these experiments.² In order to better understand the orientation of these molecules on the surface and their influence on binding to a target receptor, selective coupling and characterization of the molecule is needed. Here in we report the ability to couple peptides directly to the polymer surface with high selectivity and in some cases, without the use of protecting group strategies. Further, we can utilize this technique to quantify the amount of peptide on the array to optimize binding studies.

¹Fellet, M. S.; Bartels, J. L.; Bi, B.; Moeller, K. D. J. Am. Chem. Soc. 2012 , 134, 16891

²Hu, L.; Graaf, M. D.; Moeller, K. D. J. Electrochem. Soc. 2013 , 160 (7) G3020-G3029