We report a surfactant-free method for the synthesis of beaded manganese oxide (Mn₂O₃) nanofibers by electrospinning and their application in supercapacitors. Beaded morphology of the fibers by annealing was observed through electron microscopic analysis. High crystalline nature and lattice strain of the fibers were verified by X-ray analysis and high resolution transmission electron microscopy (HRTEM). X-ray photoelectron spectroscopy and Raman Spectroscopy techniques were used to characterize the Mn₂O₃ nanofibers. Electrochemical properties of Mn₂O₃ nanofibers for use as electrodes in supercapacitors were investigated using cyclic voltammetry, galvanostatic charge/discharge, and electrochemical impedance spectroscopy in a 0.5 M Na₂SO₄ aqueous electrolyte. The specific capacitance of the Mn₂O₃ beaded nanofibers was found to be 358 Fg^-1 at a current density of 0.5 Ag^-1. Lattice strain-induced ionic and electronic defects enhanced the surface properties of the Mn₂O₃ nanofibers, thereby improving the electrochemical properties for supercapacitor applicaions.