Metal oxide semiconductors at the nanometric scale have attracted considerable attention in recent years. Cerium oxide (CeO₂) is one such metal oxide semiconductor that has received significant attention due to its catalytic and free radical scavenging properties and finds applications in medicine, catalysis and sensing. Here CeO₂ was prepared by electrodeposition technique on a fluorine doped tin oxide (FTO) substrate. For electrodeposition, cerium nitrate and dimethyl sulfoxide (DMSO) were used. Several CeO₂ samples were prepared with varying amount of DMSO. The current density and temperature of deposition was 2 mA/cm² and 70°C respectively. The fabricated CeO₂ samples were characterized by scanning electron microscopy (SEM), X-ray diffraction analysis (XRD) and UV-visible spectroscopy. Additionally, the CeO₂ samples were tested for glucose sensing. The enzymatic glucose biosensor was prepared by immobilizing glucose oxidase (GOx) enzyme on the CeO₂ samples. The amperometric current response of the glucose biosensor was recorded at every addition of glucose. Furthermore, electrochemical impedance spectroscopic (EIS) method was used to study the charge transfer in the fabricated biosensors. Finally, a comparison of different CeO₂ biosensors was carried out.