Lithium Sulfur (Li-S) battery has a theoretically high specific capacity of 1675 mAh/g, which one of the most promising energy source for electric vehicles (EVs), hybrid electric vehicles (HEVs). However, Li-S batteries have problems resulting from the polysulfides dissolution into the electrolyte, volumetric extension of sulfur and insulating property of elemental sulfur. These causes make the loss of active materials and the capacity fading phenomenon. Previous studies show adding the interlayer is a simple and effective way for improving electrical performance of lithium sulfur batteries. In this research, we synthesize the catalyst-embedded interlayer by using catalyst-synthesized multi-walled carbon nanotubes. To confirm the catalyst on the multi-walled carbon nanotubes, transmission electron microscopy and X-ray diffraction are used. Cells were tested at the current densities of 0.2 C-rate with 1.7V to 2.8V. The electrochemical properties of catalyst-embedded interlayer are improved rather than the pristine cell.