Exosomes are nanoscale heterogeneous vesicles that are released by different cells. These vesicles plays significant role in intercellular communications, transport of proteins, RNA, and other molecular informations. In the last decade, researchers have shown substantial interest in this field as there is a lack of specific methodology to isolate and detect them. Currently, the exact science behind the major standard techniques of isolation of exosomes is not clearly understood. Thus, limitations with low yield and poor quality exosomes compromise further molecular analysis for diagnosis. The ultracentrifugation method of isolation of exosomes is time consuming, laborious, infrastructure intensive and may lack specificity. Therefore, a lot of challenges are existing in this field in order to develop next generation affinity-based technologies to capture the exosomes selectively and use them for further diagnosis at the clinical level.
The two different sensing platforms, developed and tested for sensing of exosomes are the ex-situ gold (Au) nano-islands on glass substrates and the in-situ prepared silver (Ag) - polydimethylsiloxane (PDMS) nano-composite. The gold platform is fabricated by depositing colloidal gold on glass by the thermal convection method, followed by morphology tuning of the formed nanoparticles by annealing. The second is the nano-composite platform developed by the in-situ synthesis of silver ions present in the silver nitrate solution and the curing agent present in the PDMS polymer. In both cases, the capture and detection of exosomes is based on the strong affinity of heat shock proteins contained by exosomes and a polypeptide called Vn96, specially synthesized for this purpose. The Vn96 peptide targets canonical heat shock proteins that are present on the surface of exosomes. The Vn96 - based exosomes-capture method is further validated for downstream analyses, clinical compatibility, and liquid biopsy assays (biomarker and mutation detection) and platform-versatility using cell-culture conditioned media and human body fluids as sources of exosomes. Vn96 provides multiple advantages over currently-available methods for exosomes isolation: the scalability, quality, platform versatility, and cost-effectiveness.
By using the gold platform, biotinylated Vn96 peptide is bound onto the streptavidin-coated Au nano-islands, and the subsequent steps of binding of nano-sized vesicles (exosomes) are monitored through the localized surface plasmon resonance (LSPR) band of Au. The sensing process was modelled, taking into account the characteristics of the nano-island structure. It is found that the results of the sensing process depend on the two major steps: the molar ratios of streptavidin to biotin-PEG-Vn96 and, the final step, the capture of exosomes by the biotin-PEG-Vn96 complex. The Ag-PDMS platform is used in a similar way and the shift of the Ag LSPR band is monitored after each sensing step.
It is found that the bio sensitivity of the ex-situ synthesized gold/glass platform is considerably higher than that of the in-situ synthesized Ag-PDMS nanocomposite and, consequently, this platform is much more performant for the sensing of exosomes. Two principal reasons were identified in order to account for this difference. It is thought that, because of the low temperature of annealing of Ag-PDMS, contrary to the nano-islands of gold, a non-suitable morphology is formed. It has been demonstrated that nano-island structures have a higher sensitivity due to their morphological characteristics. On the other hand, due to the in-situ formation mechanism, a large proportion of the surface Ag particles will diffuse inside the polymer layer, that is, they will not be available anymore for sensing. The morphology of Au nano-islands and Ag-PDMS composite were investigated by SEM and the LSPR techniques are discussed in the paper.