The mercaptohexanol passivation layer plays an important role for electrochemical DNA sensing, particularly when the effects of electric field are crucial to the detection scheme as is the case in electrochemical melting. The mercaptohexanol layer, or some other alkyl thiol layer is almost universally used to remove non-specifically absorbed DNA from the surface and prevent further non-specific absorption. Physisorbed oligonucleotides that are not removed by the passivation layer are expected to have ill-defined interaction energies with the surface and will be expelled from the surface at potentials and rates different than the hybridized target oligonucleotides. Additionally, a uniform mercaptohexanol layer is necessary for a uniform electric field. In this work, we examine the extent to which non-specific absorption occurs even when the mercaptohexanol layer is present. We examine how non-specific absorption effects the melting behavior, including the melting potential, melting rate, and the reproducibility. Finally, we compare various methods for mitigating non-specific absorption. These methods include the use of surfactants during electrode modification, applications of potential pulse sequences to destabilize and remove physisorbed oligomers, and the use of elevated temperatures.