In this study, Ni-doped CuSe nanowires (NWs) is prepared by by using a vacuum hydraulic pressure injection process by using anodic aluminum oxide (AAO) template assisted. The prepared steps are including: the first is nanotubular template fabricated by anodization process. Second, enlarged the tube diameter of the template by a chemical etching process. Third, Ni-doped CuSe bulk is prepared by melting the mixed element powders of Cu, Se and Ni with a sealed quartz tube. After homogenizing the bulk, the operated chamber was vacuumed and heated up to melt the Ni-doped CuSe bulk and press the liquefied Ni-doped CuSe into the AAO template then cool the sample to room temperature. The last, the AAO were removed with chromic acid + phosphoric acidsolution at 60 °C for 40 minute then cleaned with de-ionized water to get the products that stored in alcohol solution for further characterization. As-grown Ni-doped CuSe nanowires were studied by scanning electron microscope (SEM), , X-ray diffractometer (XRD) transmission electron microscope (TEM), Raman spectroscopy cathodoluminescence spectroscopy and superconducting quantum interference device (SQUID). The SEM result shows the nanowire is 100 nm and 10~30 µm in diameter and length, respectively. The TEM and XRD results confirm the product is Ni-doped CuSe nanowire with <5 at% Ni. CL spectra demonstrated that the emission of Ni-doped CuSe nanowires at ~600 nm (2.1 eV) and could be change with varied Ni concentration. The magnetic properties of Ni-doped CuSe nanowires are analyzed with SQUID and shows the Curie temperature of product is above room temperature. The unique optical and magnetic properties of AAO template-assisted grown Ni-doped CuSe nanowires could be potential application of spintronic device.