Our interest lies in using enzymes to develop biosensors to be employed in studying neurochemical processes occurring in the brain. We will discuss expression and characterization of three different wild type enzymes and their mutants (Glycine oxidase, D-aspartate oxidase, D-amino acid oxidase). Enzyme expression and purification will be completed through standard biochemistry techniques while activity characterization will be assessed using spectrophotometric assays. Upon expression and characterization, these enzymes will then be used to develop enzymatic bioelectrochemical sensors which will be employed for in vitro single cell and in-vivo animal model studies of neurochemical processes.