Electroformation is an important preparing method for giant liposomes. However, it’s a complex process and may be influenced by numerous factors, so its efficiency, size homogeneity and repeatability are still required to be improved.

In this work, all main steps of the giant liposome electroformation were analyzed. For several different kinds of lipid molecules, the lipid concentration, lipid film formation style, and the electrical signals were discussed for higher efficiency and repeatability. Furthermore, a collection method was proposed, in which the formed giant liposomes could be sorted and collected. Finally, a microchip integrated the electroformation and collection parts was developed, in which the preparation and collection of conforming to the requirements giant liposomes can be done simply and efficiently.