Fuel cells has been developed as an alternative energy source, with the advantage of using renewable fuels like alcohols obtained from biomass. Among the 12 most important building blocks from biomass is sorbitol, capable of transferring up to 26 electrons in its complete electro-oxidation. The existing literature about the sorbitol electro-oxidation reaction (SOR) is few and mostly focused on platinum alone materials in acid medium, and the proposed SOR pathway leads to glucose and then follows the glucose electro-oxidation pathway. Since there is no report concerning the effect of bimetallic nanoparticles for the SOR, we decided to test the effect of the bimetallic composition of platinum-gold nanoparticles in alkaline medium, not only for sorbitol, but also for the reported by-products; gold was chosen due to its excellent electroactivity towards glucose oxidation. The highest current density was achieved by the catalysts Pt₃₀Au₇₀ with 2.16 mA/cm², while the lowest peak potential was obtained by Pt alone at -0.03 V/NHE. The addition of gold to the bimetallic composition also change the electrochemical behavior, from various oxidation signals at low potentials for high Pt loadings to a single oxidation signal at higher potentials for low Pt loadings.