2026
Artificial Cilia for Microfluidics Particle Capture

Tuesday, 2 October 2018: 14:55
Universal 17 (Expo Center)
S. Hanasoge, A. Alexeev (Georgia Institute of Technology), M. Erickson (University of Georgia), P. J. Hesketh (Georgia Institute of Technology), and Y. R. Ortega (University of Georgia)
Microorganisms use hair like cilia to perform various biophysical functions. Particularly they use cilia to manipulate fluid flow and generate currents that enable their feeding. Natural cilia are also used to sort particles before feeding. In this work, we demonstrate artificial cilia capable of capturing particles on its surface. Magnetic filaments are used as cilia, which extend away from the substrate and into the microfluidic channel. A sample of fluid containing the target bacteria is introduced into the channel. The cilia are actuated to perform bio-mimetic oscillations. The motions of the artificial cilia enhances fluid mixing and therefore increases the probability of contacting the target cells. The antibody immobilized cilia surface captures the target bacteria upon contact. We demonstrate the capture of Salmonella bacteria on the surface of the cilia, from a sample from washing of leafy greens. The results presented in this work demonstrate the feasibility of antibody functionalized cilia to perform capture. The cells are selectively shown to attach to the surface of the cilia. This mechanism can be extended to pre-concentrate of other target cells.