Regeneration of Enzymatic Layer on Layer-By-Layer Assembled Biosensor Interfaces

Tuesday, May 13, 2014
Grand Foyer, Lobby Level (Hilton Orlando Bonnet Creek)
Y. Zhang, M. Arugula, and A. Simonian (Auburn University)
Previously, we reported an approach employing layer-by-layer (LbL) assembly of bio-functionalized multi-walled carbon nanotubes (MWCNT) on glassy carbon electrode for electrochemical detection of pesticides. In this study, to have a better understanding of self-assembled layers, surface morphology and element composition were characterized with Atomic Force Microscopy (AFM) and Scanning Electron Microscopy coupled with Energy-dispersive X-ray Spectroscopy (SEM/EDS). To further manipulate the versatile layer-by-layer assembly process, the possibility to regenerate the enzymatic layer in the layer-by-layer assembled bio-functionalized MWCNT Interfaces was investigated. Different approaches including direct chemical treatment, such as acid/alkali with different pH value, as well as inserting intermediate layer, such as conductive polymer PEDOT/NaPSS were explored. Such a strategy to achieve controllable assembly/disassembly of the layers is of great significance to enhance the biosensing system for long-term utility and/or multi-enzyme detection.