Electrochemical Characterization of Attached Marine Biofilms for Application in MFC on Glassy Carbon/Carbon Nanotubes Scaffold

Glassy carbon electrode modified with multiwalled carbon nanotubes (MWCNT) and chitosan (Chit) is tested for the biofilm attachment and electron transfer by using electrochemical techniques. This work describes the electrochemical and high surface resolution of multiwalled carbon nanotubes (MWCNT) coupled to chitosan by using Cyclic Voltamperometry (CV) and Electrochemical Impedance Spectroscopy (EIS). Confocal microscopy is used to characterize the growth of thin biofilms onto this 3D macroporous scaffolds and to corroborate the insights determined by electrochemical tecnniques. CV and EIS also reveal features that are consistent with detections of multiple redox-active species appearing in this biofilm and associated to electron transfers. These techniques depicts specifics quantifiable characteristic and defined electron transfer processes as a function of potential, electrode material, growth phase for biofilms derived from marine sediments and marine waters.