Wavelet transform (WT)-based noise suppression is preferentially selected as the basic methodology in this research. The OVT is considered as the non-stationary signal with different time interval and transient phenomena, and is used as an input signal in the WT. These rules basically implemented the multi-resolution analysis (MRA) that have a vigorous function of both time and frequency localization. Based on this MRA, an original OVT (with noise) is divided into two components such as low- and high-frequency component, An and Dn. Two components have respectively corresponding coefficients, approximate- aj,k and detailed coefficients dj,k, for implementation of the decomposition and reconstruction processes in the MRA. Among two coefficients, because of abrupt change during a short period of time of noise, the detailed coefficient dj,k should be adjusted to suppress noise included in an original OVT. In general, the performance on noise suppression is intimately linked with decomposition and reconstruction levels in the MRA. Specifically, this research different from previous studies is to show the clear comparison of the performance on noise suppression based on two rules of the multi-level wavelet transform (MLWT) and single connected-unit level wavelet transform (SC-ULWT) from the OVT of a PEM fuel cell. For MLWT-based noise suppression, without use of detail component Dn, only approximation component decomposed at the previous level is considered as an input at the next decomposition level. The reconstruction process is vice versa. On the contrary, the SC-ULWT performed the MRA and noise suppression at every unit decomposition and reconstruction levels. As it were, the noise suppressed OVT at the previous unit level is used an input at the next unit level. This rule applied n unit level WT that suppressed sensing of noisy at every steps and connected in series. This research compared the performance on noise suppression between the MLWT and SC-ULWT by the signal-to-noise (SNR) ratio calculation. The comparative analysis on noise suppression between two rules clearly showed in this research. For reference, the PEM fuel cell for this research was made by the ‘Materials and Electro-Chemistry Laboratory’ in Inha University.