This study investigated the combination of Aganonerion Polymorphum leaf extraction (APLE) and Titania nanoparticles (TNPs) as main and synergy inhibitor for steel in ethanol fuel blend. APLE has been extracted from soxhlet apparatus, and the APLE components were checked by Fourier transform infrared spectroscopy. TNPs with various sizes were synthesized by sol-gel route from tetraisopropyl orthotitanate precursor. TNPs sizes were calculated by Scherrer’s formula from X-ray diffraction pattern and confirmed by scanning electron microscopy (SEM). Immersion test was taken in simulated fuel environment followed ASTM standards. Electrochemical impedance spectroscopy indicated that APLE established a good adherent protective layer onto the steel surface, which would have been better with TNPs. Tafel fitting from potentiodynamic measurement had a good agreement with EIS data and shown higher corrosion potential and polarization resistance, as well as lower corrosion current density and corrosion rate of the co-inhibiting system. X-ray photoelectron spectroscopy manifested the significant decrease of Fe peaks intensity and the significant increase of C_1s and Ti_2p peaks from specimens without to one with the inhibitors, that confirmed the inhibiting effectiveness of APLE and TNPs. Finally, SEM images designated smoother surface of specimens submerged in the co-inhibiting system that strengthen those remarks above.