(Invited) Simultaneous Ionic Current and Potential Detection of Biomolecules and Nanoparticles By a Multifunctional Nanopipette

Wednesday, 31 May 2017: 11:20
Eglinton Winton (Hilton New Orleans Riverside)
J. He (Florida International University)
Nanopore sensing based technologies have made significant progress for biomedical applications. In recent years, multimode sensing by multifunctional nanopores shows the potential to greatly improve the sensitivity and selectivity of traditional resistive-pulse sensing method. In this talk, we will show that two label-free electric sensing modes can work cooperatively to detect biological entities, such as biomolecules and biological nanoparticles, in ionic solution by a multifunctional nanopipette. The multifunctional nanopipettes containing both nanopore and nanoelectrode (NE) at the tip are fabricated quickly and cheaply. We will discuss the detection mechanism and demonstrate that the ionic current and local electrical potential changes can be detected simultaneously during the translocation of individual entities. With the combination of ionic current and potential measurements, we are able to detect not only the translocation of single entity but also the motions of entities outside the nanopore entrance, revealing rich information of biological entities.