Electrolytic Deposition of Doxorubicin-Chitosan Composites on Post Hydroxyapatite Coated Titanium Alloy for Localizing Tumor Treatments

Wednesday, 31 May 2017
Grand Ballroom (Hilton New Orleans Riverside)
S. K. Yen, S. R. Hsu, Y. M. Cheng (National Chung Hsing University), Y. L. Lai (China Medical University Hospital, Taiwan), and C. C. Lin (Taichung Armed Force General Hospital, Taiwan)
In order to reduce the side effects caused by the chemotherapy of cancer, various target treatments and drug-delivery systems have been investigated. In this study, the doxorubicin-chitosan composite was deposited on the post hydroxyapatite coated Ti alloy for the tumor treatment by sustaining release of localized drug to achieve the inhibition or apoptosis of cancer cells. In the experiment, cathodic polarization tests coupled with electrochemical reactions were analyzed to speculate the deposition mechanism of doxorubicin, UV-visible spectrometer to measure the doxorubicin loading and release, field emission scanning electron microscope (FESEM) to observe the surface morphology, Fourier transform infrared spectroscopy (FTIR) to characterize the chemical bonds of composite, and X-ray diffractometry (XRD) the crystal structures. The MTT assay was carried out to analyze the toxicity of the drug to cells. It is concluded that doxorubicin-chitosan composites can be successfully deposited on the titanium alloy by the electrochemical method, while the post hydroxyapatite coated specimen with high porosity can enhance the drug loading and reveal the complete drug release. Besides, the electrochemical deposition does not vary any chemical bonding or destroy any medical function of doxorubicin, revealing a practical method for drug loading.

Keywords: Doxorubicin, Chitosan, Hydroxyapatite coated Ti alloy, Localize, Tumor.