Redox Responsive Fluorescent Probe Development for Super Resolution and Fluorescence Lifetime Microscopy

Wednesday, 31 May 2017
Grand Ballroom (Hilton New Orleans Riverside)
J. Woodcock (MML, NIST), E. Choudhary (CNST, NIST, Maryland NanoCenter, University of Maryland), B. Jones (MML, NIST), J. Marr, A. Kumar (CNST, NIST, Maryland NanoCenter, University of Maryland), J. W. Gilman, S. J. Stranick (MML, NIST), J. A. Liddle, and V. A. Szalai (CNST, NIST)
Redox-sensing in aqueous solutions using super resolution fluorescence or lifetime microscopy has been applied extensively to image metabolic events in biological systems. Typically, such probes are designed to fluoresce following reaction with (or capture of) a particular analyte, for example, reactive oxygen species or a redox-active metal ion. Using such probes as a guide, we have designed and characterized new redox-responsive fluorescent probes intended for fluorescence-based imaging of aqueous electrochemical systems. We present solution spectroelectrochemical characterization of the parent dye molecules as well as initial optical microscopy characterization of these redox-sensitive fluorophores attached to a thermally-responsive polymer gel. Using thermal-control of polymer matrix thickness, the surface density of dye on the electrode can be modulated and imaged using fluorescence lifetime with super resolution microscopy.