Protein biomarkers are becoming increasingly important in diagnosis and monitoring of diseases. Paper-based test strip is a promising technique for sensing protein biomarkers in point-of-care (POC) applications. This talk will demonstrate our effort to develop a paper-based test strips for detection of Traumatic Brain Injury (TBI) protein biomarker in blood plasma by utilizing employing surface-enhanced Raman scattering (SERS) for signal transduction. The detection of limit (LOD) of this paper test strip for TBI protein biomarker is lower than the cut off value that is present in patient blood plasma. This paper-based lateral flow device has a potential application in POC setting.