Recently we dramatically simplified the fusion pore size extraction procedure (without sacrificing its accuracy) so that it can be easily implemented by the experimentalists, e.g. in spreadsheet programs. This advance allow us to address a larger data set of spikes obtained at chromaffin cells and reveal changes in fusion pores topology under modified conditions (osmotic stress, membrane lipid modification) with respect to control conditions. Of high interest was the finding that in all considered cases the fusion pore radii was never larger 30 nm, that is much smaller to the average radius of the chromaffin cell vesicle 156 nm. Taking into account significant size of the data set (more than 1000 spikes) this questions the ‘inevitable full fusion’ paradigm and statistically support a mode of exocytosis where the pore size is significantly smaller the vesicle size .
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