Diabetes is a serious and one of the growing health care problems worldwide and is associated with the oxidative DNA damage resulting increased level of metabolites such as 8-hydroxydeoxyguanosine (8-OHdeGuo) and 8 – hydroxyguanine (8-OHGua) in biological fluids. In the present studies efforts have been made for the development of a simple, fast and reliable method for the analysis of these products formed by the oxidative DNA damage in body fluids. An electrochemical study at gold nanoparticles attached single walled carbon nanotubes modified pyrolytic graphite sensor has been employed for studying the oxidation of these compounds with an aim to develop a method for the self diagnosis of diabetes. The fabricated sensor was characterized by using FE-SEM, AFM and Impedance studies and the effective surface area of the sensor surface was found to enhance significantly in comparison to the bare surface. The oxidation of both the compounds occurred in a pH dependent process in square wave voltammetry and the electrode reaction followed adsorption controlled pathway. Under optimum conditions linear calibration curve for 8-OHGua is obtained over the concentration range 0.01 – 10.0 nM in phosphate buffer of pH 7.2 and detection limit and sensitivity of 5.0 (± 0.1) pM and 4.9 (± 0.1) μA nM⁻¹, are observed respectively. Similarly 8-OHdeGuo exhibited linear dynamic range of 0.005 – 20 nM and detection limit of 1.0 (± 0.1) pM was observed. The electrode exhibited an efficient catalytic response with good reproducibility and stability. The common metabolites present in urine such as ascorbic acid, uric acid and dopamine did not interfere in the determination. The method has been found selective and successfully used for the determination of these compounds in urine samples of diabetic patients.