Food-borne bacteria contamination in food and water has become an important topic for food industries as well as common people. Escherichia coli is one of the common infectious bacteria and the maximum acceptance of E. coli in drinking water in Canada is 0/100 mL. Current traditional detection techniques such as ELISA and PCR are time-consuming, tedious and expensive. To develop more rapid and effective detection methods, several studies had reported the preference of cell imprinting technique in the biosensor applications ^(2,4). However, the sensitivity and the accessibility of the biosensors remain a challenge. In this regard, we have developed a capacitive biosensor based on a layer by layer, boronic acid doped polyaniline methacrylic based molecular imprinted polymers (MIPs) on a carbon nanotube conductive film. The tailor-made MIPs have been found to be highly selective affinity to E. coli. Highly miniaturized (the size of 1.5 x 1.5 cm²) and inexpensive, the fabricated sensor schematically depicted below is currently being hyphenated to cellular phones through a blue tooth, can be deployed for on site remote analysis of pathogens in drinking water and food analysis. The sensor performance was impressive with a wide dynamic range of four orders of magnitude and low limit of detection of 36 CFU/mL for E. coli detection. Moreover, by simply tailoring the molecular receptors, the sensor can be employed in the detection of other pathogenic bacteria such as Staphylococcus spp. and Salmonella spp. in food and water.