2445
Comparison of Pathogens Capture By Different Bio-Receptors Immobilized Biomolecular Filter in a Large Volume of Liquid

Wednesday, 16 May 2018: 17:20
Room 303 (Washington State Convention Center)
S. Du (Materials Research & Education Center, Auburn University), I. H. Chen (Material Research & Education Center, Auburn University), Y. Liu (Materials Research & Education Center, Auburn University), J. Xi, X. Lu (Material Research & Education Center, Auburn University), S. Horikawa (Auburn University), T. S. Huang (Poultry Science, Auburn University), S. J. Suh (Department of Biological Sciences, Auburn University), and B. A. Chin (Material Research & Education Center, Auburn University)
Foodborne illness is a common public health problem because food can be contaminated by pathogens at any point from farm to dinner table. This paper shows a comparison of capturing a small quantity of a specific bacterial pathogen in a large volume of liquid using a biomolecular filter immobilized with different bioreceptors. The filter consists of support frames, which are made of a soft magnetic material, and solenoid coils for magnetization/demagnetization of the frames. The biomolecular filter is a planar, multi-layered arrangement of strip-shaped magnetoelastic (ME) biosensors with bioreceptor immobilization. These ME biosensors are magnetically held and arrayed on the filter frames. The pathogen of interest in a large volume of liquid is captured by the bioreceptor immobilized on the ME biosensors, when a large volume of liquid passes through. At the same time, non-specific debris can be passed through the biomolecular filter, in order to avoiding a common clogging issue from conventional bead filters. In this work, E2 phage and antibody, are immobilized each on ME biosensors to capture Salmonella Typhimurium in a large volume of water. The capture efficiencies and effects of multiplication of filter layers with E2 phage and the antibody on Salmonella capture efficiency will be discussed.