Effect of Varying Polyethylene Glycol Chain Length on Enzyme Activity

It is well-established in research literature that ceria nanoparticles (CNPs) are able to undergo redox reaction with several oxygen-containing species in single or double electron transfers. The oxidase-mimetic ability of these particles, specifically, has found use in several biosensor devices. In this study we sought to characterize and optimize the use of ceria nanoparticles coated in polyethylene glycol (PEG) for use in biosensors. Specifically, molecular weight of adsorbed polymer was varied, particles were allowed to age in solution, and each solution was assayed for catalase activity. Interestingly, this study revealed that different polymer chain lengths of PEG influence self-assembly of particles in solution, thus varying oxidase activity.