Highly Selective Detection of Glucose, Cholesterol and Urea with Integrated ZnO Nanorods Field-Effect Transistors Array Biosensors

Nanotechnology revolution has led to the nanofabrication of sensor devices for rapid and specific identification of chemical/biological species. However, the development of multiplexed nanoscale biosensor for simultaneous detection of different analytes still remains a major challenge at the nanotechnology frontier. It is well recognized that diabetes mellitus is a metabolic disorder resulting in an abnormal blood glucose level and activation of several metabolic pathways related to inflammation and apoptosis events. We have developed ZnO nanorods (NRs) based, integrated field-effect transistors (i-FETs) array biosensor with simultaneously immobilizing GOx, ChOx and Ur enzymes on three different ZnO NRs arrays. In this lecture, we reoprt a novel straightforward approach for simultaneous and highly selective detection of multi-alanytes (i.e., glucose, cholesterol and urea) with the i-FETs array biosensor without interference in each sensor response. Compared to analytically measured data, performance of the i-FETs array biosensor is found to be highly reliable for rapid detection of multi-analytes in mice blood, serum and blood smaples of diabetic dogs. The development of an integrated, low-cost ZnO NRs i-FETs array biosensor will produce quick detection under critical patient conditions, early identification of disease/disorder, and also have an enormous impact on the future generations.