A fine needle type glucose sensor, which has sensing region on the tip of a fine tapered electrode, was prepared using tapered PEEK tube with platinum-iridium alloy wire. Glucose oxidase was immobilized on the surface of Pt-Ir electrode using the combination of electrodeposition and electropolymerization procedures. The obtained sensor showed good response with sufficient linear relationship between response current and glucose concentration. Long-term stability of the obtained electrode was evaluated for 35 days. Constant response current was obtained for approximately 1 month.