In this presentation, we will discuss development of molecularly imprinted polymer (MIP) films selective towards some prospective biomarkers of renal dysfunction, namely, inosine and lipocaline-2. This artificial recognition units can easily be integrated with extended gate-field effect transistors (EG-FETs) allowing for development of selective and robust chemosensors for the target analytes [1, 2].

For the last decade, chemically-sensitive field-effect transistors (ChemFETs) have been applied as transduction units in chemosensors. They offer numerous advantages, such as, ease of miniaturization, possibility of high-throughput sensing, high sensitivity, important detection limit, and possibility to adjust sensitivity according to specific needs. Various reports have described great variety of such devices for determination of different analytes of interest, ranging from ions, gases, small molecules, macromolecules and even whole viruses [3, 4]. Selectivity of the devised chemo- and biosensors is typically introduced by modifying gate region of the FET with suitable recognition units selective towards the analyte. In this regard, MIPs are attractive artificial recognition units offering good sensitivity and selectivity combined with robustness and resistance to harsh experimental conditions [5]. Here, we have made an attempt to combine the selectivity and robustness of MIPs with sensitivity and amplification features of EG-FET transducers to devise sensitive and selective chemosensors for selected bioanalytes.

Bibliography

1. Z. Iskierko et al., Biosens. Bioelectron., 74, 526, (2015).
2. Z. Iskierko et al. ACS Appl. Mater. Interfaces, 8, 19860, (2016).
3. Y. Cheng, et al., Biosens. Bioelectron., 26, 4538, (2011).
4. F. Patolsky, et al., Proc. Natl. Acad. Sci. U.S.A., 101, 14017 (2004).
5. P. S. Sharma, et al. Trends Anal. Chem., 51, 146, (2013).