One major push in sensor development is production of very inexpensive, easy to use sensors that require minimal external equipment. Microfluidic Paper-based Analytical Devices (mPADs) have received significant attention because they meet these requirements and can carry out a wide range of chemical and biochemical assays. Detection with mPADs is normally done with colorimetric reactions but these reactions can be limited in sensitivity and selectivity. Improvements in colorimetric detection as well as adaption of more sensitive detection methods are needed to meet the growing demand for point-of-need diagnostic applications. This talk will focus on recent developments from the Henry laboratory using electrochemistry with mPADs with applications in point-of-need sensing. Incorporating electrodes into mPADs is a key element for achieving targeted selectivity and sensitivity. Examples of carbon and metallic electrodes will be discussed as well as an innovative dual electrode system that enables simultaneous electrochemistry at two different solution conditions. Example applications will be shown for both environmental and clinical sensing. For environmental sensing, stripping voltammetry has been adapted to mPADs to measure trace metals in aerosolized particulate matter. In this application, the benefits of low volume and ease of sample preparation have enabled screening of hundreds of samples from wearable aerosol samplers to be analyzed for exposure. For biological sensing, example applications for detection bacteria and viruses will be shown. Bacteria can be detected by measuring the activity of enzymes produced by the bacteria or through electrochemical immunoassays. Viruses can be detected using electrochemical immunosensors incorporated into mPADs. Recent results for both applications will be discussed.