Lithium-sulfur (Li-S) battery with high theoretical energy density of 2600 Wh/kg, expected to be next generation battery. In reality Li-dendrite growth, polysulfide formation, shuttling effect, low conductivity of sulfur and low active material loading become major challenges to develop Li-S battery technology. To overcome these problems we have tried a bilayer separator, which consists of low dimensional molybdenum oxide (MoO₃) nanorods on one side and polymer on other side to prevent the migration of polysulfides. The low dimensional transition metal oxide was prepared by simple physical grinding process. 10 wt% solution was prepared in isopropyl alcohol (IPA) and spray coated onto one side of the celgard. Initial capacity of 1185 mAh/g was achieved at 0.5 C (1C=1675mAh/g) and at the end of 500 cycles 80% initial discharge capacity was retained with degradation rate of 0.04% per cycle. Rate capability of the Li-S battery using MoO₃ coated separator was measured starting with 0.5 C rate followed by 1 C, 2 C, 3 C, 4 C and finally, return to 0.5 C and the test illustrates that the Li-S battery possesses very good rate capability by showing 93.21% retention of initial discharge capacity.